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Handbook of Methods in Aquatic Microbial Ecology is the first comprehensive compilation of 85 fundamental methods in modern aquatic microbial ecology. Each method is presented in a detailed, step-by-step format that allows readers to adopt new methods with little difficulty. The methods represent the state of the art, and many have become standard procedures in microbial research and environmental assessment. The book also presents practical advice on how to apply the methods. It will be an indispensable reference for marine and freshwater research laboratories, environmental assessment laboratories, and industrial research labs concerned with microbial measurements in water.-

Content: Introduction (J.E. Hobbie). Isolation of Living Cells: Isolation and Enumeration of Anaerobic and Microaerophilic Bacteria in Aquatic Habitats (M.J. Ferrara-Guerrero, D.G. Marty, and A. Bianchi). Isolation and Culture of Hyperthermophilic Bacteria from Marine and Freshwater Habitats (J.A. Baross). Isolation of Psychrophilic Bacteria (R.Y. Morita). Isolation and Characterization of Bacteriocytes from a Bivalve-Sulfur Bacterium Symbiosis (S.C. Hand and A.E. Anderson). General Techniques for the Isolation and Culture of Marine Protists from Estuarine, Littoral, Psammolittoral, and Sublittoral Waters (J.J. Lee). Long-Term Culture of Marine Benthic Protists (P.G. Carey). Behavior and Bioenergetics of Anaerobic and Microaerobic Protists (B.J. Finlay). Culturing Free-Living Marine Phagotrophic Dinoflagellates (E.J. Lessard). Enrichment, Isolation, and Culture of Free-Living Heterotrophic Flagellates (D.A. Caron). Determination of Pressure Effects on Flagellates Isolated from Surface Waters (C.M. Turley). Isolation, Cloning, and Axenic Culturing of Marine Ciliates (A.T. Soldo and S.A. Brickson). Isolation and Laboratory Culture of Marine Oligotrichous Ciliates (D.J. Gifford). Extraction of Protists in Aquatic Sediments via Density Gradient Cetrifugation (D.M. Alongi). Identification, Enumeration, and Diversity: Statistical Analysis of Direct Counts of Microbial Abundance (D.L. Kirchman). Enumeration and Isolation of Viruses (C.A. Suttle). Total Count of Viruses in Aquatic Environments (G. Bratbak and M. Heldal). Improved Sample Preparation for Enumeration of Aggregated Aquatic Substrate Bacteria (M.I. Velji and L.J. Albright). Direct Estimates of Bacterial Numbers in Seawater Samples without Incurring Cell Loss Due to Sample Storage (C.M. Turley). Total and Specific Bacterial Counts by Simultaneous Staining with DAPI and Fluorochrome-Labeled Antibodies (K.A. Hoff). Use of RFLPs for the Comparison of Marine Cyanobacteria (S.E. Douglas). Use of High-Resolution Flow Cytometry

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